Isolation of human IgA and IgM from normal serum using polyethylene glycol precipitation and affinity chromatography.

Abstract

A simple method is described for the preparation of highly purified IgA and IgM from small volumes of human serum. Enriched IgM and IgA fractions were prepared by precipitation with 7% (w/v) and 14% (w/v) polyethylene glycol respectively. This was followed by affinity chromatography and gel filtration. The final recovery of both IgA and IgM was… (More)

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@article{Cripps1983IsolationOH, title={Isolation of human IgA and IgM from normal serum using polyethylene glycol precipitation and affinity chromatography.}, author={Allan W. Cripps and Siew-Chin Neoh and I. J. Smart}, journal={Journal of immunological methods}, year={1983}, volume={57 1-3}, pages={197-204} }