Isolation of hofbauer cells from human term placentas with high yield and purity.

@article{Tang2011IsolationOH,
  title={Isolation of hofbauer cells from human term placentas with high yield and purity.},
  author={Zhong-hua Tang and Serkalem Tadesse and Errol R. Norwitz and Gil Mor and Vikki M Abrahams and S. A. Guller},
  journal={American journal of reproductive immunology},
  year={2011},
  volume={66 4},
  pages={336-48}
}
PROBLEM  Placental villus macrophages (i.e., Hofbauer cells, HBCs) were identified more than 100 years ago. Alterations in their numbers and characteristics are associated with several complications of pregnancy. Although HBCs have previously been isolated and cultured, there is no consensus methodology to obtain these cells with high yield and purity for in vitro studies. METHOD OF STUDY  Hofbauer cells were isolated from human term placentas using protocols in which cytotrophoblasts (CTs) and… CONTINUE READING