Isolation of high-quality RNA from polyphenol-, polysaccharide- and lipid-rich seeds.

@article{Birti2006IsolationOH,
  title={Isolation of high-quality RNA from polyphenol-, polysaccharide- and lipid-rich seeds.},
  author={Simona Birti{\'c} and Ilse Kranner},
  journal={Phytochemical analysis : PCA},
  year={2006},
  volume={17 3},
  pages={144-8}
}
Seed polyphenols, polysaccharides and lipids often interfere with or degrade RNA, restricting its yield and quality. Existing RNA isolation methods specifically alleviate one or two of these challenges, but are usually designed for a single species rather than for a broad biodiversity. Many protocols also do not eliminate chromosomal DNA, which causes false positives in gene expression studies. The method reported here addresses all of the above challenges. The concentration of the phenol… CONTINUE READING