Isolation of high-chlorophyll-fluorescence mutants ofArabidopsis thaliana and their characterisation by spectroscopy, immunoblotting and Northern hybridisation

@article{Meurer1996IsolationOH,
  title={Isolation of high-chlorophyll-fluorescence mutants ofArabidopsis thaliana and their characterisation by spectroscopy, immunoblotting and Northern hybridisation},
  author={J{\"o}rg Meurer and Karin Meierhoff and Peter Westhoff},
  journal={Planta},
  year={1996},
  volume={198},
  pages={385-396}
}
Thirty-four recessive photosynthetic mutants of the high-chlorophyll-fluorescence (hcf) phenotype have been isolated by screening 7700 M2 progenies of ethyl methane sulfonate-treated seeds ofArabidopsis thaliana. Most of the mutants isolated were found to be seedlinglethal, but could be grown on sucrose-supplemented media. Chlorophyll (Chl) fluorescence induction, absorption changes in the reaction-centre chlorophyll of PS I (P700) at 830 nm and Chla/Chlb ratios were recorded in order to probe… CONTINUE READING
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