Isolation of high-affinity monomeric human anti-c-erbB-2 single chain Fv using affinity-driven selection.

@article{Schier1996IsolationOH,
  title={Isolation of high-affinity monomeric human anti-c-erbB-2 single chain Fv using affinity-driven selection.},
  author={Robert Schier and Jacqueline Bye and Gerald Apell and Adrian M. McCall and Gregory P. Adams and Magnus Malmqvist and Louis M. Weiner and James D Marks},
  journal={Journal of molecular biology},
  year={1996},
  volume={255 1},
  pages={28-43}
}
The use of antibodies to target tumor antigens has had limited success, partially due to the large size of IgG molecules, difficulties in constructing smaller single chain Fv (scFv) antibody fragments, and immunogenicity of murine antibodies. These limitations can be overcome by selecting human scFv directly from non-immune or semi-synthetic phage antibody libraries; however, the affinities are typically too low for therapeutic application. For hapten antigens, higher-affinity scFv can be… CONTINUE READING

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