Isolation of hepatitis C virus RNA from serum for reverse transcription-PCR.

@article{Nolte1994IsolationOH,
  title={Isolation of hepatitis C virus RNA from serum for reverse transcription-PCR.},
  author={Frederick S Nolte and C Thurmond and Peter S. R. Mitchell},
  journal={Journal of clinical microbiology},
  year={1994},
  volume={32 2},
  pages={
          519-20
        }
}
  • Frederick S Nolte, C Thurmond, Peter S. R. Mitchell
  • Published in
    Journal of clinical…
    1994
  • Biology, Medicine
  • Standard multistep extraction and isolation of RNA for hepatitis C virus (HCV) reverse transcription (RT)-PCR are impractical for routine use in clinical laboratories. We compared three simple commercially available methods for RNA isolation (RNAzol B, TRISOLV, and ULTRASPEC; Biotecx Laboratories, Houston, Tex.) and a total nucleic acid isolation method (IsoQuick; MicroProbe Corp., Garden Grove, Calif.) for the recovery of HCV RNA from sera obtained from 12 viremic patients for RT-PCR. RNAzol B… CONTINUE READING

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