Isolation of glutathione-deficient mutants of the yeast Saccharomyces cerevisiae.

  title={Isolation of glutathione-deficient mutants of the yeast Saccharomyces cerevisiae.},
  author={Manfred Kistler and Karl Heinz Summer and Friederike Eckardt},
  journal={Mutation research},
  volume={173 2},
Glutathione-deficient (gsh-) mutants of the yeast Saccharomyces cerevisiae were isolated after UV treatment using MNNG as selective agent. For genetic and biochemical characterization 5 mutant strains were chosen which exhibited considerably decreased residual GSH contents varying from 2 to 6% of the wild-type levels. All 5 isolates showed a 2:2 segregation of the gsh-:GSH+ phenotypes alluding to a monogenic recessive mutation. Complementation analysis indicates that all gsh- mutants belong to… CONTINUE READING

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