Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase.

@article{Huang2012IsolationOG,
  title={Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase.},
  author={Lanxiang Huang and Ewelina Garbulewska and Kazuaki Sato and Yuichi Kato and Masahiro Nogawa and Goro Taguchi and Makoto Shimosaka},
  journal={Journal of bioscience and bioengineering},
  year={2012},
  volume={113 3},
  pages={293-9}
}
Chitiniphilus shinanonensis type strain SAY3(T) is a strongly chitinolytic bacterium, originally isolated from the moat water in Ueda, Japan. To elucidate the chitinolytic activity of this strain, 15 genes (chiA-chiO) coding for putative chitin-degrading enzymes were isolated from a genomic library. Sequence analysis revealed the genes comprised 12 family 18 chitinases, a family 19 chitinase, a family 20 β-N-acetylglucosaminidase, and a polypeptide with a chitin-binding domain but devoid of a… CONTINUE READING

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ChitosanNo subtypeChitin
The recombinant protein rChiN catalyzed an endo - type cleavage of N - acetyl - d - glucosamine oligomers , and also degraded insoluble chitin and soluble chitosan ( degree of deacetylation of 80% ) .
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