Isolation of fully synthetic promoters for high-level gene expression in Corynebacterium glutamicum.

@article{Yim2013IsolationOF,
  title={Isolation of fully synthetic promoters for high-level gene expression in Corynebacterium glutamicum.},
  author={Sung Sun Yim and Seul Ji An and Misuk Kang and Jinho Lee and K. J. Jeong},
  journal={Biotechnology and bioengineering},
  year={2013},
  volume={110 11},
  pages={2959-69}
}
Corynebacterium glutamicum is an important industrial organism that is widely used in the production of amino acids, nucleotides and vitamins. To extend its product spectrum and improve productivity, C. glutamicum needs to undergo further engineering, including the development of applicable promoter system. Here, we isolated new promoters from the fully synthetic promoter library consisting of 70-bp random sequences in C. glutamicum. Using green fluorescent protein (GFP) as a reporter, highly… CONTINUE READING