Isolation of fetal DNA from nucleated erythrocytes in maternal blood.

  title={Isolation of fetal DNA from nucleated erythrocytes in maternal blood.},
  author={Diana W. Bianchi and A. P. F. Flint and M F Pizzimenti and Joan H. M. Knoll and Samuel A. Latt},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={87 9},
Fetal nucleated cells within maternal blood represent a potential source of fetal genes obtainable by venipuncture. We used monoclonal antibody against the transferrin receptor (TfR) to identify nucleated erythrocytes in the peripheral blood of pregnant women. Candidate fetal cells from 19 pregnancies were isolated by flow sorting at 12 1/2-17 weeks gestation. The DNA in these cells was amplified for a 222-base-pair (bp) sequence present on the short arm of the Y chromosome as proof that the… CONTINUE READING


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