Isolation of endothelial cells from human term placental villi using immunomagnetic beads.

Abstract

Chorionic villi excised from freshly delivered human term placentae and small endothelial cell aggregates were released from them by the sequential use of collagenase and trypsin. The endothelial cells were further isolated by rosetting with magnetic polystyrene beads which were coated with QB END/40, the endothelial-specific monoclonal antibody (mAb) to… (More)

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