Isolation of endo A cDNA from mouse 8-cell stage embryos.


To analyse the species of genes expressed in a cleavage stage mouse embryo, we have constructed a cDNA library containing 3.0 x 10(5) independent clones from about 2 x 10(3) embryos at the 8-cell stage of development. Endo A cDNA prepared from parietal yolk sac endoderm like PYS-2 cells was used to screen the library. Southern blot analyses using the endo A sequence as a probe and restriction mapping analyses revealed that four independent recombinants had been inserted endo A sequence. Sequencing data of these clones showed that endo A mRNA present in the 8-cell stage embryo is identical to that of parietal yolk sac endoderm cells.

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@article{Nozaki1988IsolationOE, title={Isolation of endo A cDNA from mouse 8-cell stage embryos.}, author={Masashi Nozaki and K Murata and Takehito Morita and Aizo Matsushiro}, journal={Biochemical and biophysical research communications}, year={1988}, volume={154 3}, pages={890-4} }