Isolation of denatured proteins and peptides by high-performance liquid chromatography. Effect of different perfluorinated acids, column length and large-pore supports.

Abstract

The utilization of high-performance liquid chromatography for the isolation of peptides and proteins is extended by the demonstration that trifluoroacetic acid, pentafluoropropionic acid and heptafluorobutyric acid are effective peptide and protein solvents and as components of a mobile phase alter both the absolute and relative retention times of eluting… (More)

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@article{Mahoney1982IsolationOD, title={Isolation of denatured proteins and peptides by high-performance liquid chromatography. Effect of different perfluorinated acids, column length and large-pore supports.}, author={Walter C. Mahoney}, journal={Biochimica et biophysica acta}, year={1982}, volume={704 2}, pages={284-9} }