Isolation of corneal epithelium with Dispase II or EDTA. Effects on the basement membrane zone.

Abstract

An ultrastructural and immunohistochemical comparison was made between the effects of Dispase II and EDTA on the basement membrane zone of corneal epithelium. The comparison was made on intact corneas as well as on freed epithelial sheets and remnant stromas that had been separated using either the enzyme or chelator. At the ultrastructural level… (More)

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@article{Spurr1985IsolationOC, title={Isolation of corneal epithelium with Dispase II or EDTA. Effects on the basement membrane zone.}, author={Stephen J. Spurr and Ilene K. Gipson}, journal={Investigative ophthalmology & visual science}, year={1985}, volume={26 6}, pages={818-27} }