Isolation of centromeric-tandem repetitive DNA sequences by chromatin affinity purification using a HaloTag7-fused centromere-specific histone H3 in tobacco

@article{Nagaki2011IsolationOC,
  title={Isolation of centromeric-tandem repetitive DNA sequences by chromatin affinity purification using a HaloTag7-fused centromere-specific histone H3 in tobacco},
  author={Kiyotaka Nagaki and Fukashi Shibata and Asaka Kanatani and Kazunari Kashihara and Minoru Murata},
  journal={Plant Cell Reports},
  year={2011},
  volume={31},
  pages={771-779}
}
The centromere is a multi-functional complex comprising centromeric DNA and a number of proteins. To isolate unidentified centromeric DNA sequences, centromere-specific histone H3 variants (CENH3) and chromatin immunoprecipitation (ChIP) have been utilized in some plant species. However, anti-CENH3 antibody for ChIP must be raised in each species because of its species specificity. Production of the antibodies is time-consuming and costly, and it is not easy to produce ChIP-grade antibodies. In… CONTINUE READING

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