Isolation of cDNAs for perilipins A and B: sequence and expression of lipid droplet-associated proteins of adipocytes.

@article{Greenberg1993IsolationOC,
  title={Isolation of cDNAs for perilipins A and B: sequence and expression of lipid droplet-associated proteins of adipocytes.},
  author={Andrew S Greenberg and Judith J Egan and Sheree A. Wek and Malcolm C Moos and Constantine Londos and Alan R. Kimmel},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1993},
  volume={90 24},
  pages={12035-9}
}
The major cAMP-dependent protein kinase (A-kinase) substrate in adipocytes is perilipin, a protein found exclusively at the surface of the lipid storage droplets. Using anti-perilipin serum, we have isolated two related classes of full-length coding cDNAs, designated perilipin A and B, from a rat adipocyte cDNA expression library. The two cDNAs derive from two mRNA species that arise by differential splicing. The mRNAs are predicted to encode perilipins A and B, proteins of 517 aa (56,870 Da… CONTINUE READING