Isolation of cDNA fragments hybridizing to rat brain-specific mRNAs.

@article{Esposito1990IsolationOC,
  title={Isolation of cDNA fragments hybridizing to rat brain-specific mRNAs.},
  author={Francesco Maria Esposito and Rosario Ammendola and Angela Duilio and F. S. Costanzo and Mara Giordano and Nicola Zambrano and Paul D'Agostino and Tommaso Russo and Filiberto Cimino},
  journal={Developmental neuroscience},
  year={1990},
  volume={12 6},
  pages={373-81}
}
A cDNA minilibrary in pUC18 has been generated from 3-month-old rat brains. Two hundred clones were randomly selected and sequenced. Comparison with a nucleic acid and protein data bank revealed a number of cDNA fragments not homologous to any published sequence. Northern blot analyses of some of these clones yielded 5 brain-specific cDNA fragments. The… CONTINUE READING