Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast.

@article{Singh1991IsolationOC,
  title={Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast.},
  author={Mohan B Singh and Tertius Hough and P Theerakulpisut and A Avjioglu and Sally Davies and Penelope M.C. Smith and Palmer Taylor and Richard J. Simpson and Larry D Ward and James McCluskey},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 4},
  pages={1384-8}
}
We have identified a major allergenic protein from rye-grass pollen, tentatively designated Lol pIb of 31kDa and with pI 9.0. A cDNA clone encoding Lol pIb has been isolated, sequenced, and characterized. Lol pIb is located mainly in the starch granules. This is a distinct allergen from Lol pI, which is located in the cytosol. Lol pIb is synthesized in pollen as a pre-allergen with a transit peptide targeting the allergen to amyloplasts. Epitope mapping of the fusion protein localized the IgE… CONTINUE READING

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