Isolation of cDNA coding for an ubiquitous membrane protein deficient in high Na+, low K+ stomatocytic erythrocytes.

@article{Stewart1992IsolationOC,
  title={Isolation of cDNA coding for an ubiquitous membrane protein deficient in high Na+, low K+ stomatocytic erythrocytes.},
  author={Gordon W. Stewart and B E Hepworth-Jones and Jeffrey N. Keen and Bipin C Dash and Annette C Argent and Colin M Casimir},
  journal={Blood},
  year={1992},
  volume={79 6},
  pages={1593-601}
}
Human red blood cells (RBCs) that are deficient in an integral membrane-associated protein ("stomatin") of apparent molecular mass 31 Kd show a catastrophic increase in passive membrane permeability to the univalent cations Na+ and K+ and are stomatocytic in shape. We have purified this protein from normal RBC membranes and isolated a cDNA clone coding for it. The deduced protein sequence is unrelated to that of any known ion-transport-related protein. Selective solubilization studies using… CONTINUE READING