Isolation of cDNA clones encoding T cell-specific membrane-associated proteins

@article{Hedrick1984IsolationOC,
  title={Isolation of cDNA clones encoding T cell-specific membrane-associated proteins},
  author={Stephen M. Hedrick and David I. Cohen and Ellen A. Nielsen and Mark M. Davis},
  journal={Nature},
  year={1984},
  volume={308},
  pages={149-153}
}
Of 10 distinct cloned DNA copies of mRNAs expressed in T lymphocytes but not in B lymphocytes and associated with membrane-bound polysomes, one hybridizes to a region of the genome that has rearranged in a T-cell lymphoma and several T-cell hybridomas. These characteristics suggest that it encodes one chain of the elusive antigen receptor on the surface of T lymphocytes. 

Sequence relationships between putative T-cell receptor polypeptides and immunoglobulins

Comparison of the sequence of a cloned T cell-specific cDNA with those of cross-reacting cloned cDNAs isolated from a thymocyte library indicates the presence of variable, constant and joining

Complete primary structure of a heterodimeric T-cell receptor deduced from cDNA sequences

TLDR
Two related, but distinct cDNA clones have been isolated and sequenced from a functional murine cytotoxic T-lymphocyte clone and it is concluded that these genes code for the two subunits of the heterodimeric antigen receptor on the surface of the T cell.

A third type of murine T-cell receptor gene

TLDR
A new species of T-cell receptor cDNA clone whose predicted amino acid sequence has homology to variable, constant, joining and diversity segments of immunoglobulins and T- cell receptors is isolated.

Somatic recombination in a murine T-cell receptor gene

TLDR
Analysis of the sequence components of this rearranged gene indicates the presence of separate variable, diversity and joining region elements analogous to those of heavy-chain immunoglobulins, which support the belief that the products of this locus are involved in antigen recognition by T cells.

cDNA cloning of the B cell membrane protein CD22: a mediator of B-B cell interactions

TLDR
Northern blot analysis revealed low-level BL-CAM mRNA expression in unactivated tonsillar B cells, which was rapidly increased after B cell activation with Staphylococcus aureus Cowan strain 1 and phorbol myristate acetate, but not by various cytokines, including interleukin 4 (IL-4), IL-6, and gamma interferon.

Expression of T-cell receptor by a mouse monoclonal antigen-specific suppressor T-cell line.

The antigen receptor expressed by mouse T lymphocytes is composed of a disul- fide-linked dimer of about 85K, resolved under reducing conditions in two sub-units with an apparent Mr of approximately

cDNA cloning and sequence of MA) associated with human T-cell differ, (subtractive hybridization/ontogeny/T-cell activation/transport prol

TLDR
The predicted configuration of the MAL protein resembles the structure of integral proteins that form pores or channels in the plasma membrane and that are believed to act as transporters of water-soluble molecules and ions across the lipid bilayer.

Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex

TLDR
The complete amino acid sequence of one of the polypeptide chains of the human T3/T-cell receptor complex, the T3 glycoprotein (T3-δ-chain) was deduced from cDNA clones derived from HPB-ALL cells and a human T-cell clone.
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References

SHOWING 1-10 OF 47 REFERENCES

Sequence relationships between putative T-cell receptor polypeptides and immunoglobulins

Comparison of the sequence of a cloned T cell-specific cDNA with those of cross-reacting cloned cDNAs isolated from a thymocyte library indicates the presence of variable, constant and joining

Mouse immunoglobulin D: construction and characterization of a cloned delta chain cDNA.

TLDR
The hybridization selection technique has been used to show that this cDNA clone specifically binds only mRNA that can be translated into immunoprecipitable delta chains, and shows homology with the C delta 3 of a human myeloma protein.

T and B cells that recognize the same antigen do not transcribe similar heavy chain variable region gene segments

TLDR
T and B lymphocytes responding to GAT do not utilize similar VH gene segments, and the presence of idiotypic determinants on T lymphocytes does not necessarily imply close structural similarity between T and B cell antigen receptors.

Three T cell hybridomas do not contain detectable heavy chain variable gene transcripts

TLDR
It is concluded that VH gene segments are not likely to encode the antigen-specific receptor in the cells the authors tested and should not be encoded in the cDNA libraries constructed from one suppressor and two helper T cell hybridomas.

Membrane-bound ribosomes of myeloma cells. IV. mRNA complexity of free and membrane-bound polysomes

TLDR
Analysis of the sequence complexity, frequency distribution, and template activity of free (F) and membrane-bound (MB) polysomal mRNA populations of MOPC 21 (P3K) mouse myeloma cells finds that Ig L mRNA, representing approximately 5% of totalpolysomal poly(A)+ RNA, is one of the most abundant MB mRNAs.

The isolation of cloned cDNA sequences which are differentially expressed in human lymphocytes and fibroblasts.

TLDR
A direct estimation of the abundance of five sequences in lymphocyte cDNA demonstrated that clones can be detected by such a procedure if they represent 0.2% or greater of the original cDNA population.

Thy-1 cDNA sequence suggests a novel regulatory mechanism

TLDR
The isolation and characterization of a cDNA clone encoding the rat thymus Thy-1 antigen is reported but it is found that the DNA sequence ends prematurely at a position corresponding to amino acid 103, which appears to be a complete transcript.

Contribution of antigen-presenting cell major histocompatibility complex gene products to the specificity of antigen-induced T cell activation

Previous studies from our laboratory showed that B 10.A mice are high responders to pigeon cytochrome c fragment 81-104, whereas’B 10.A(5R) mice are low responders. In the present studies, the