Isolation of brush-border membrane from the rabbit descending colon epithelium. Partial characterization of a unique K+-activated ATPase.

@article{Gustin1981IsolationOB,
  title={Isolation of brush-border membrane from the rabbit descending colon epithelium. Partial characterization of a unique K+-activated ATPase.},
  author={Michael Gustin and David B. P. Goodman},
  journal={The Journal of biological chemistry},
  year={1981},
  volume={256 20},
  pages={
          10651-6
        }
}
The mechanisms of ion movement across the apical membrane of the colon have previously been investigated only in intact tissue. To investigate these mechanisms directly, we have undertaken the isolation and characterization of the apical brush-border membrane of the rabbit descending colon. The purification protocol consists of an initial isolation of single epithelial cells after dissociation of the mucosal layer in EDTA, a high pH (8.3), low ionic strength homogenization of the cells, and… CONTINUE READING
Highly Cited
This paper has 29 citations. REVIEW CITATIONS

Citations

Publications citing this paper.
Showing 1-10 of 19 extracted citations

H-K-ATPase type 2: relevance for renal physiology and beyond.

American journal of physiology. Renal physiology • 2014

Prechylomicron transport vesicle: isolation and partial characterization.

The American journal of physiology • 1999
View 2 Excerpts
Method Support

Targeted disruption of the mouse villin gene does not impair the morphogenesis of microvilli.

Developmental dynamics : an official publication of the American Association of Anatomists • 1998

Gastrointestinal Regulatory Peptides

Handbook of Experimental Pharmacology • 1993