Isolation of brush-border membrane from the rabbit descending colon epithelium. Partial characterization of a unique K+-activated ATPase.

@article{Gustin1981IsolationOB,
  title={Isolation of brush-border membrane from the rabbit descending colon epithelium. Partial characterization of a unique K+-activated ATPase.},
  author={Michael Gustin and David B. P. Goodman},
  journal={The Journal of biological chemistry},
  year={1981},
  volume={256 20},
  pages={
          10651-6
        }
}

• Michael Gustin, David B. P. Goodman
• Published 1981 in The Journal of biological chemistry

The mechanisms of ion movement across the apical membrane of the colon have previously been investigated only in intact tissue. To investigate these mechanisms directly, we have undertaken the isolation and characterization of the apical brush-border membrane of the rabbit descending colon. The purification protocol consists of an initial isolation of single epithelial cells after dissociation of the mucosal layer in EDTA, a high pH (8.3), low ionic strength homogenization of the cells, and… CONTINUE READING

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