Isolation of botulinolysin, a thiol-activated hemolysin, from serotype D Clostridium botulinum: A species-specific gene duplication in Clostridia.

  title={Isolation of botulinolysin, a thiol-activated hemolysin, from serotype D Clostridium botulinum: A species-specific gene duplication in Clostridia.},
  author={Tomonori Suzuki and Thomas Nagano and Koichi Niwa and Shingo Mutoh and Masataka Uchino and Motohiro Tomizawa and Yoshimasa Sagane and Toshihiro Watanabe},
  journal={Microbiological research},


Production, purification, and characterization of botulinolysin, a thiol-activated hemolysin of Clostridium botulinum
The purified hemolysin had a lethal effect in mice and was cytotoxic for some cultured cells: its 50% lethal dose in mice was 310 ng, and the 50% cytot toxic dose for Vero cells was 120 ng/ml.
Molecular Characterization of GroES and GroEL Homologues from Clostridium botulinum
Southern and Northern blot analyses indicate that the groESL operon is encoded on the genomic DNA of D-4947 as a single copy, and not on that of its specific toxin-converting phage.
Characterization of Toxin Complex Produced by a Unique Strain of Clostridium botulinum Serotype D 4947
A new method is established to separate the unique D-4947 NT from the complex, which will yield valuable information on structure of botulinum toxin, which is indistinguishable from the parent L-TC with respect to toxicity, hemagglutination activity and gel filtration profile.
Clostridium botulinum group III: a group with dual identity shaped by plasmids, phages and mobile elements
The completion of the genome of C. botulinum group III has revealed it to be a genome with dual identity, which belongs to the pathogenic species C. Botulinum, but as a genotypic species it should also include C. novyi and C. haemolyticum.
Simple Method for Detection of Clostridium botulinum Type A to F Neurotoxin Genes by Ploymerase Chain Reaction
This PCR‐based detection method can be used for the rapid diagnosis of botulism and was established to detect each type of neurotoxin genes of Clostridium botulinum types A to F by employing the oligonucleotide primer sets corresponding to special regions of the light chains of the neurotoxins.
Neurotoxin Gene Profiling of Clostridium botulinum Types C and D Native to Different Countries within Europe
Interestingly, only BoNT mosaics, types C-D and D-C, were found in naturally contaminated samples whatever their animal origin and their geographical location, and further investigations should now be performed in order to check that mosaic types dominate in Europe and that acquisition of mosaic types helps in survival or adaptation to particular niche.
Phylogeny and taxonomy of the food‐borne pathogen Clostridium botulinum and its neurotoxins
  • Collins, East
  • Biology
    Journal of applied microbiology
  • 1998
The current state of knowledge of the phylogenetics of the species Cl. botulinum and its neurotoxins is reviewed, and a view is presented that a nomenclature based rigidly on BoNT production is no longer tenable.
Genetic characteristics of toxigenic Clostridia and toxin gene evolution.
  • M. Popoff, P. Bouvet
  • Biology
    Toxicon : official journal of the International Society on Toxinology
  • 2013
Alveolysin, the thiol-activated toxin of Bacillus alvei, is homologous to listeriolysin O, perfringolysin O, pneumolysin, and streptolysin O and contains a single cysteine
The deduced amino acid sequence of alveolysin shows that alveolarsin shares homologies with listeriolysin O, perfringolysin O, pneumolysin, and streptolysinO, and a single cysteine in the conserved peptide sequence ECTGLA WEWWR.