Isolation of bone cell clones with differences in growth, hormone responses, and extracellular matrix production

  title={Isolation of bone cell clones with differences in growth, hormone responses, and extracellular matrix production},
  author={Jane E. Aubin and Johan N. M. Heersche and Mervyn J. Merrilees and Jaro Sodek},
  journal={The Journal of Cell Biology},
  pages={452 - 461}
Clones of nontransformed hormone-responsive bone cells have been isolated in vitro from mixed cell populations of fetal rat calvaria. In several independent isolations, microscopically visible colonies appeared at plating efficiencies of 5-10% of the starting cell numbers. Of these clones, approximately 10% grew to mass populations which could be assayed for a number of growth and biochemical properties. Although some similarities existed among the clones, they could be distinguished from each… CONTINUE READING
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