Isolation of bacterial type IV machine subassemblies.

  title={Isolation of bacterial type IV machine subassemblies.},
  author={Mayukh K Sarkar and Seyyed I. Husnain and Simon Jakubowski and Peter J Christie},
  journal={Methods in molecular biology},
The bacterial type IV secretion systems (T4SSs) deliver DNA and protein substrates to bacterial and eukaryotic target cells generally by a mechanism requiring direct contact between donor and target cells. Recent advances in defining the architectures of T4SSs have been made through isolation of machine subassemblies for further biochemical and ultrastructural analysis. Here, we describe a protocol for isolation and characterization of VirB protein complexes from the paradigmatic VirB/VirD4… CONTINUE READING

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