Isolation of axolemma-enriched fractions from bovine central nervous system.


Axolemmal fractions are isolated by discontinuous gradient centrifugation of purified myelinated axons which have been osmotically shocked to yield axons, myelin and axolemma. The axolemmal fractions are enriched in surface membrane marker enzymes such as Na(+),K(+)-activated ATPase, 5'-nucleotidase and acetylcholinesterase. Mixing experiments with labeled… (More)


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