Isolation of an inhibitor of actin polymerization from human polymorphonuclear leukocytes.


A large proportion of actin in extracts of human polymorphonuclear leukocytes is unpolymerized in the presence of 0.1 M KCl, a condition expected to promote maximal assembly of purified actin. Raising of the KCl concentration increases the extent of actin polymerization in leukocyte extracts, and, at KCl concentrations greater than or equal to 0.3 M, the… (More)