Isolation of an inducible amidase from Pseudomonas acidovorans AE1.

@article{Alt1975IsolationOA,
  title={Isolation of an inducible amidase from Pseudomonas acidovorans AE1.},
  author={Juliane Alt and K. S. Krisch},
  journal={Journal of general microbiology},
  year={1975},
  volume={87 2},
  pages={
          260-72
        }
}
A bacterial strain, AEI, which hydrolysed acetanilide, was isolated from soil and identified as Pseudomonas acidovorans. Numerous amides, esters and enzyme inhibitors were tested as amidase inducers. Phenacetin was chosen as inducer for the large scale cultivation of these organisms because it was less toxic to the bacteria than acetanilide. The induction increased the enzymic activity 250-fold. In comparison, the type culture strain of P. acidovorans, ATTCCI5668, had no amidase activity which… CONTINUE READING

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