Isolation of adipose-derived stem cells by using a subfractionation culturing method.

@article{Yi2014IsolationOA,
  title={Isolation of adipose-derived stem cells by using a subfractionation culturing method.},
  author={Tacghee Yi and Wang-Kyun Kim and Joon-Seok Choi and Seung Yong Song and Juhee Han and Ji Hye Kim and Won Serk Kim and Sang Gyu Park and Hyun-Joo Lee and Yun kyoung Cho and Sung-Joo Hwang and Sun Uk Song and Jong-Hyuk Sung},
  journal={Expert opinion on biological therapy},
  year={2014},
  volume={14 11},
  pages={
          1551-60
        }
}
OBJECTIVE Adipose-derived stem cells (ASCs) isolated from subcutaneous adipose tissue have been tested in clinical trials. However, ASCs isolated by enzyme digestion and centrifugation are heterogeneous and exhibit wide variation in regenerative potential and clinical outcomes. Therefore, we developed a new method for isolating clonal ASCs (cASCs) that does not use enzyme digestion or centrifugation steps. RESEARCH DESIGN AND METHODS In addition to cell surface markers and differentiation… CONTINUE READING
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