Isolation of a transforming sequence from a human bladder carcinoma cell line

  title={Isolation of a transforming sequence from a human bladder carcinoma cell line},
  author={Chiaho Shih and Robert A. Weinberg},

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Transforming genes in human tumors
Preliminary results indicating that the T24 bladder carcimoma oncogene is highly related to the transforming gene of BALB‐MSV, an acute transforming retrovirus is reported.
Mechanism of activation of human ras genes cloned from a gastric adenocarcinoma and a pancreatic carcinoma cell line.
A rasN gene from a human gastric adenocarcinoma which efficiently induced both morphological transformation and tumorigenicity of NIH3T3 cells in a transfection assay is identified and sequence analysis reveals a G to A change within codon 12, which is presumably responsible for its biological activity.
Characterization of a human colon/lung carcinoma oncogene
A characterization of an oncogene found originally to be present in DNA of the SW480 colon carcinoma cell line is presented and its presence in an activated form in the genome of several types of human tumour cell lines as well as in biopsy tissue from an adenocarcinoma of the large bowel is identified.
Localization of the normal allele of T24 human bladder carcinoma oncogene to chromosome 11
It is shown here that the T24 oncogene, which is of human origin and closely related to the onc genes of BALB and Harvey murine sarcoma viruses, is carried on chromosome 11 in normal cells.
Characterization of human transforming genes from chemically transformed, teratocarcinoma, and pancreatic carcinoma cell lines.
The results show that the transforming gene activated in the teratocarcinoma cell line is identical to the neuroblastoma transforming gene and that the Transformation gene of the pancreatic carcinoma cellline is a human homologue of rasK.
Transformation of normal homologous cells by a spontaneously activated Ha-ras oncogene.
Under well-defined experimental conditions, a spontaneously activated Ha-ras oncogene from a naturally occurring tumor was able to independently transform normal, homologous cells to a malignant phenotype.
Oncogenes in solid human tumours
It is reported here that unmanipulated human solid tumours, including carcinomas of the colon, lung and pancreas and an embryonal rhabdomyosarcoma, also contain dominant transforming genes, and several other human tumour cell lines, including those established from carcinomasOf the colon (A2233), lung (A427 and A2182), gall bladder and urinary bladder, possess the same oncogene.
Production of recombinant rat viruses as a method of oncogene isolation in coculture medium.
The emergence of virus containing mos from mos DNA-mediated normal rat kidney transformants demonstrated "rescue" of the active cellular oncogene by the rat leukemia virus, and this coculture system seems to facilitate "res rescue" of oncogenes functioning in the tumor and transformed cells.
Mouse skin carcinomas induced in vivo by chemical carcinogens have a transforming Harvey-ras oncogene
It is reported here that high molecular weight DNA from transplanted squamous cell carcinomas induced by sequential treatment of mouse skin with initiators and promoters of carcinogenesis causes morphological transformation of NIH/3T3 fibroblasts at high frequency.


Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts
DNAs obtained from human, rabbit and mouse bladder carcinomas lines, a lung carcinoma line and rat neuroblastoma and mouse glioma lines, are able to induce transformation of NIH3T3 cells on transfection.
Passage of phenotypes of chemically transformed cells via transfection of DNA and chromatin.
These experiments prove that in five mouse and rat cell lines the chemically induced phenotype is encoded in DNA, and the sequences specifying the transformed phenotype behave as a dominant allele in the NIH3T3 recipient cells.
Markers of neoplastic transformation in epithelial cell lines derived from human carcinomas.
Five epithelial tumor cell lines derived from different human bladder tumors had many of the structural and ultrastructural features of both the original tumor and the tissue of origin, even though the cells had been through many in vitro passages.
Activation of related transforming genes in mouse and human mammary carcinomas.
High molecular weight DNAs of five tumors induced by mouse mammary tumor virus (MMTV), two mouse mammary tumors induced by a chemical carcinogen, and one human mammary tumor cell line (MCF-7) were
Isolation and localization of DNA segments from specific human chromosomes.
A library of genomic DNA segments has been constructed from the DNA of a somatic cell hybrid carrying a portion of human chromosome 11 on a Chinese hamster ovary cell background, and using a nucleic acid hybridization technique that distinguishes human and Chinese hamsters interspersed, repetitive DNA, this approach promises implications for human genetics generally, for the human genetic diseases, and possibly for understanding of gene regulation in normal and abnormal differentiation.
Transforming activity of human tumor DNAs.
  • T. Krontiris, G. Cooper
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1981
It is suggested that dominant mutations or gene rearrangements can result in the activation of cellular transforming genes in some human tumors.