Isolation of a reovirus from a broiler chicken flock with high early mortality.

Abstract

A reovirus was isolated from a flock of 12,000 broiler chickens which experienced a total mortality of 6.3% up to 35 days. The reovirus produced large syncytia in primary chicken kidney cell cultures with eosinophilic intracytoplasmic inclusion body formation. Infected cells reacted with fluorescein conjugated specific anti-reovirus serum. Negatively strained virions had a double shelled appearance with overall diameter of 65 to 70 nm. The nucleic acid had ten discrete segments with electrophoretic mobilities as would be expected for a reovirus.

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@article{Saifuddin1989IsolationOA, title={Isolation of a reovirus from a broiler chicken flock with high early mortality.}, author={M Saifuddin and C R Wilks and N H Christensen and Z F Fu and M Rice}, journal={New Zealand veterinary journal}, year={1989}, volume={37 1}, pages={12-4} }