Isolation of a putative phospholipase c gene of drosophila, norpA, and its role in phototransduction

@article{Bloomquist1988IsolationOA,
  title={Isolation of a putative phospholipase c gene of drosophila, norpA, and its role in phototransduction},
  author={Brian T. Bloomquist and R. D. Shortridge and Stephan Schneuwly and M. H. Perdew and Craig Montell and Hermann Steller and Gerald M. Rubin and William L. Pak},
  journal={Cell},
  year={1988},
  volume={54},
  pages={723-733}
}
Severe norpA mutations in Drosophila eliminate the photoreceptor potential and render the fly completely blind. Recent biochemical analyses have shown that norpA mutants lack phospholipase C (PLC) activity in the eye. A combination of chromosomal walking and transposon-mediated mutagenesis was used to clone the norpA gene. This gene encodes a 7.5 kb RNA that is expressed in the adult head. In situ hybridizations of norpA cDNA to adult tissue sections show that this gene is expressed abundantly… Expand
Specific molecular alterations in the norpA‐encoded phospholipase C of Drosophila and their effects on electrophysiological responses in vivo
TLDR
It is shown that the specific PLC activity is a key factor determining the photoreceptor sensitivity; the catalytic activity and response termination are separable functions of PLC; and a mutation in the putative Gα‐interacting C2 domain causes a preferentially strong defect in latency. Expand
Multiple Subtypes of Phospholipase C Are Encoded by the norpA Gene of Drosophila melanogaster(*)
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The norpA gene of Drosophila melanogaster encodes a phosphatidylinositol-specific phospholipase C that is essential for phototransduction, and its products are found abundantly in retina and a second subtype of norPA protein that is generated by alternative splicing is identified. Expand
Phospholipase C Rescues Visual Defect in norpA Mutant of Drosophila melanogaster(*)
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Western blots of head homogenates stained with NorpA antiserum show that norpA protein is restored in heads of transformed mutants, the strongest evidence thus far supporting the hypothesis that the norPA gene encodes phospholipase C that is utilized in phototransduction. Expand
Membrane association of phospholipase c encoded by thenorpA gene ofdrosophila melanogaster
TLDR
It is found that both phospholipase C activity and the norpA protein are predominantly associated with membrane fractions in heads of both light- and dark-adapted flies, indicating that the norPA protein is peripherally localized on the membrane. Expand
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It is found that the non‐retinal norpA‐PLC enzyme can substitute for its retinal counterpart, but that there is a reduced rate of repolarization of photoreceptors following intense light stimuli. Expand
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TLDR
The isolation and preliminary characterization of a new G α gene (dgq) in Drosophila is described, yielding two putative proteins that represent a new class of G α subunits because they lack both high amino acid identity with other G α proteins and the pertussis toxin ADP ribosylation site. Expand
Chapter 9 Genetic dissection of Drosophila phototransduction
TLDR
It is clear that mutations in many of the genes associated with the transduction cascade lead to retinal degeneration in both vertebrates and Drosophila, but the underlying pathological mechanisms are only poorly understood. Expand
Chromosomal localization of three mouse diacylglycerol kinase (DAGK) genes: genes sharing sequence homology to the Drosophila retinal degeneration A (rdgA) gene.
TLDR
In their efforts to consider genes mutated in Drosophila as candidates for mammalian eye disease, this work has initially determined the map position of three DAGK genes in the mouse. Expand
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