Isolation of a putative keratocyte activating factor from the corneal stroma.

@article{Musselmann2003IsolationOA,
  title={Isolation of a putative keratocyte activating factor from the corneal stroma.},
  author={Kurt Musselmann and Bradley P Kane and J. Hassell},
  journal={Experimental eye research},
  year={2003},
  volume={77 3},
  pages={273-9}
}
Fetal bovine serum has commonly been used to expand the population of keratocytes in culture. Tissue extracts, however, have also been used to grow other cell types. We prepared a DMEM/F12 extract of corneal stroma and compared the growth and morphology of collagenase-isolated keratocytes cultured in DMEM/F12, or DMEM/F12 containing either stromal extract or fetal bovine serum. Cell proliferation was measured by 3H-thymidine and BrdU incorporation as well as by DNA quantitation. The extract was… CONTINUE READING

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