Isolation of a novel tetrapeptide with opiate and antiopiate activity from human brain cortex: Tyr-Pro-Trp-Gly-NH2 (Tyr-W-MIF-1)

@article{rchegyi1992IsolationOA,
  title={Isolation of a novel tetrapeptide with opiate and antiopiate activity from human brain cortex: Tyr-Pro-Trp-Gly-NH2 (Tyr-W-MIF-1)},
  author={Judit {\'E}rchegyi and Abba J. Kastin and James E. Zadina},
  journal={Peptides},
  year={1992},
  volume={13},
  pages={623-631}
}
...

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Bovine hypothalamic tissue was extracted and purified by solid phase extraction and several reversed-phase HPLC steps. The amino acid sequence of the purified peptide was determined by Edman

Evidence for presence of Tyr-MIF-1 (Tyr-Pro-Leu-Gly-NH2) in human brain cortex.

Evidence for the presence of Tyr-MIF-1 from bovine to human brain tissue and from hypothalamus to cortex is extended and four subsequent high performance liquid chromatographic steps are described.

Interactions of Tyr-MIF-1 at opiate receptor sites