Isolation of a novel promoter for efficient protein expression by Aspergillus oryzae in solid-state culture

@article{Bando2011IsolationOA,
  title={Isolation of a novel promoter for efficient protein expression by Aspergillus oryzae in solid-state culture},
  author={Hiroki Bando and H. Hisada and Hiroki Ishida and Yoji Hata and Yoshio Katakura and Akihiko Kondo},
  journal={Applied Microbiology and Biotechnology},
  year={2011},
  volume={92},
  pages={561-569}
}
A novel promoter from a hemolysin-like protein encoding the gene, hlyA, was characterized for protein overexpression in Aspergillus oryzae grown in solid-state culture. Using endo-1,4-β-glucanase from A. oryzae (CelA) as the reporter, promoter activity was found to be higher than that of the α-amylase (amyA) and manganese superoxide dismutase (sodM) genes not only in wheat bran solid-state culture but also in liquid culture. Expression of the A. oryzae endoglucanase CelB and two heterologous… CONTINUE READING