Isolation of a novel freshwater agarolytic Cellvibrio sp. KY-YJ-3 and characterization of its extracellular beta-agarase.

@article{Rhee2010IsolationOA,
  title={Isolation of a novel freshwater agarolytic Cellvibrio sp. KY-YJ-3 and characterization of its extracellular beta-agarase.},
  author={Young Joon Rhee and Cho Rong Han and Won-Chan Kim and Do Youn Jun and In Ku Rhee and Young Ho Kim},
  journal={Journal of microbiology and biotechnology},
  year={2010},
  volume={20 10},
  pages={
          1378-85
        }
}
A novel agarolytic bacterium KY-YJ-3, producing extracellular agarase, was isolated from the freshwater sediment of the Sincheon River in Daegu, Korea. On the basis of gram-staining data, morphology, and phylogenetic analysis of the 16S rDNA sequence, the isolate was identified as Cellvibrio sp. By ammonium sulfate precipitation followed by Toyopearl QAE-550C, Toyopearl HW-55F, and Mono-Q column chromatography, the extracellular agarase in the culture fluid could be purified 120.2-fold with… 
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