Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia.

@article{Zaki2012IsolationOA,
  title={Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia.},
  author={Ali Mohamed Zaki and Sander van Boheemen and Theo M. Bestebroer and Albert D M E Osterhaus and Ron A. M. Fouchier},
  journal={The New England journal of medicine},
  year={2012},
  volume={367 19},
  pages={
          1814-20
        }
}
A previously unknown coronavirus was isolated from the sputum of a 60-year-old man who presented with acute pneumonia and subsequent renal failure with a fatal outcome in Saudi Arabia. The virus (called HCoV-EMC) replicated readily in cell culture, producing cytopathic effects of rounding, detachment, and syncytium formation. The virus represents a novel betacoronavirus species. The closest known relatives are bat coronaviruses HKU4 and HKU5. Here, the clinical data, virus isolation, and… 
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References

SHOWING 1-10 OF 29 REFERENCES
A previously undescribed coronavirus associated with respiratory disease in humans.
TLDR
The identification and characterization of a not previously recognized coronavirus obtained from an 8-mo-old boy suffering from pneumonia is described, revealing that it is most closely related to porcine epidemic diarrhea virus and HCoV 229E.
Identification of a novel coronavirus in patients with severe acute respiratory syndrome.
TLDR
The novel coronavirus might have a role in causing SARS and was detected in a variety of clinical specimens from patients with SARS but not in controls.
Characterization of a Novel Coronavirus Associated with Severe Acute Respiratory Syndrome
TLDR
Phylogenetic analyses and sequence comparisons showed that SARS-CoV is not closelyrelated to any of the previouslycharacterized coronaviruses.
Identification of a new human coronavirus
TLDR
Screening of clinical specimens from individuals suffering from respiratory illness identified seven additional HCoV-NL63-infected individuals, indicating that the virus was widely spread within the human population.
Characterization and Complete Genome Sequence of a Novel Coronavirus, Coronavirus HKU1, from Patients with Pneumonia
TLDR
The data support the existence of a novel group 2 coronavirus associated with pneumonia in humans, CoV-HKU1, from a 71-year-old man with pneumonia who had just returned from Shenzhen, China.
Coronavirus as a possible cause of severe acute respiratory syndrome
Severe acute respiratory syndrome.
TLDR
The concerted and coordinated response that contained SARS is a triumph for global public health and provides a new paradigm for the detection and control of future emerging infectious disease threats.
A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses
TLDR
A pancoronavirus degenerate primer-based method that allows the detection of all known and possibly unknown coronaviruses by RT-PCR amplification and sequencing of a 251-bp fragment of the coronavirus polymerase gene is described.
Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study
Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease.
TLDR
A sharp decrease in the rate of virus isolation during the winter of 1965-66 was noted, similar to that reported by others, and the recovery and properties of these 6 viruses form the basis of this report.
...
...