Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia.

@article{Zaki2012IsolationOA,
  title={Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia.},
  author={Ali Mohamed Zaki and Sander van Boheemen and Theo M. Bestebroer and Albert Osterhaus and Ron A. M. Fouchier},
  journal={The New England journal of medicine},
  year={2012},
  volume={367 19},
  pages={
          1814-20
        }
}
A previously unknown coronavirus was isolated from the sputum of a 60-year-old man who presented with acute pneumonia and subsequent renal failure with a fatal outcome in Saudi Arabia. The virus (called HCoV-EMC) replicated readily in cell culture, producing cytopathic effects of rounding, detachment, and syncytium formation. The virus represents a novel betacoronavirus species. The closest known relatives are bat coronaviruses HKU4 and HKU5. Here, the clinical data, virus isolation, and… 

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