Isolation of a new type C retrovirus (HTLV) in primary uncultured cells of a patient with Sézary T-cell leukaemia

@article{Poiesz1981IsolationOA,
  title={Isolation of a new type C retrovirus (HTLV) in primary uncultured cells of a patient with S{\'e}zary T-cell leukaemia},
  author={B. Poiesz and F. Ruscetti and M. Reitz and V. Kalyanaraman and R. Gallo},
  journal={Nature},
  year={1981},
  volume={294},
  pages={268-271}
}
Retroviruses have been isolated from many animal species and several have been shown to be the aetiological agents of naturally occurring leukaemias, lymphomas and sarcomas (for recent reviews see ref. 1). There is evidence that these viruses or components of them are present in humans, but the isolation and analysis of retroviruses from human cells and evidence for an association with disease have been difficult to demonstrate (see refs 2,3). We now report the isolation and characterization of… Expand
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The family of human T-lymphotropic leukemia viruses: HTLV-I as the cause of adult T cell leukemia and HTLV-III as the cause of acquired immunodeficiency syndrome.
TLDR
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HTLV-I antibodies associated with cutaneous T cell lymphoma in Denmark.
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Antibodies in human sera reactive against an internal structural protein of human T-cell lymphoma virus
TLDR
It is found that antibodies against p24 are present in human sera (including those of patient C.R. and his wife), and that these are specifically directed at HTLVCR proteins and not at cell-specific determinants—the first evidence for a specific immune response in humans against a retrovirus. Expand
Characterization and distribution of nucleic acid sequences of a novel type C retrovirus isolated from neoplastic human T lymphocytes.
A type C retrovirus (designated HTLV) recently isolated from a cell line derived from a lymph node and later from peripheral blood of a person with cutaneous T-cell lymphoma (mycosis fungoides) wasExpand
Immunological properties of a type C retrovirus isolated from cultured human T-lymphoma cells and comparison to other mammalian retroviruses
TLDR
Observations agree with immunological comparisons between reverse transcriptase of HTLV CR and other retroviruses and nucleic acid sequence homology studies which indicate that the various HTLVCR isolates represent new retrovirus found in some human T-cell neoplasias. Expand
Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma
TLDR
The number of these particle-associated proteins is consistent with the expected proteins of a retrovirus, but the sizes of some are distinct from those of most known retroviruses of the primate subgroups. Expand
Characterization of the reverse transcriptase from a new retrovirus (HTLV) produced by a human cutaneous T-cell lymphoma cell line.
Abstract The reverse transcriptase from a new retrovirus, HTLV, isolated from a human cutaneous T-cell lymphoma cell line was characterized. The enzyme is associated with high-molecular-weight RNA ofExpand
Isolation and tissue distribution of type-C virus and viral components from a gibbon ape (Hylobates lar) with lymphocytic leukemia.
TLDR
A seven-year-old male gibbon ape (Hylobates lar) with malignant lymphoma and leukemia was obtained from a colony in Bermuda, highlighting the extraordinary replication of this type of virus in gibbons and suggesting that the oral route is a likely mode of virus transmission. Expand
T-cell lines established from human T-lymphocytic neoplasias by direct response to T-cell growth factor.
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The morphologic and histochemical properties of the cell lines, the abnormal karyotype of C TCL-3, and the independent growth of CTCL-2 support the conclusion that most of these cell lines are of malignant origin. Expand
Cutaneous T-cell lymphomas: the Sézary syndrome, mycosis fungoides, and related disorders.
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It is suggested that these lymphoproliferative disorders be grouped together as "cutaneous T-cell lymphomas", because the anergy noted in patients of this group with leukemia probably is related to both decreased percentages of normal T cells and presence in the serum of macrophage migration inhibitory activity. Expand
Functional and morphologic characterization of human T cells continuously grown in vitro.
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The functional and morphologic characteristics of these cultured cells show that these cells are T cells that grow continuously in vitro, similar to those of fresh peripheral blood cells. Expand
Purification and some characteristics of human T-cell growth factor from phytohemagglutinin-stimulated lymphocyte-conditioned media.
  • J. Mier, R. Gallo
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1980
TLDR
Human T-cell growth factor, a mitogenic protein that appears in the media of cultured lymphocytes after phytohemagglutinin-stimulation, has been purified more than 400-fold from serum-free conditioned media by using a sequence of ion exchange chromatography and gel filtration, indicating that the factor is a second signal in the T- cell immune response. Expand
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