Isolation of a new cDNA clone encoding an Rh polypeptide associated with the Rh blood group system

@article{Kajii2004IsolationOA,
  title={Isolation of a new cDNA clone encoding an Rh polypeptide associated with the Rh blood group system},
  author={E. Kajii and F. Umenishi and S. Iwamoto and S. Ikemoto},
  journal={Human Genetics},
  year={2004},
  volume={91},
  pages={157-162}
}
The polymerase chain reaction (PCR) was used to amplify Rh-related cDNAs from erythroid cells cultured by the selective two-phase liquid culture system for human erythroid progenitors in peripheral blood. Direct sequencing based on PCR presents heterozygous bands. Two Rh polypeptide cDNAs have been isolated from the PCR products and tentatively designated RhPI cDNA and RhPII cDNA. Both cDNA clones have an open reading frame composed of 1251 nucleotides. The RhPI cDNA clone shows a single… Expand
28 Citations
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