Isolation of a lipopolysaccharide (LPS)-resistant mutant, with defective LPS binding, of cultured macrophage-like cells.

@article{HaraKuge1990IsolationOA,
  title={Isolation of a lipopolysaccharide (LPS)-resistant mutant, with defective LPS binding, of cultured macrophage-like cells.},
  author={Sayuri Hara-Kuge and Fumio Amano and Masahiro Nishijima and Yuzuru Akamatsu},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 12},
  pages={
          6606-10
        }
}
Lipopolysaccharide (LPS)-resistant mutants which did not respond to LPS were isolated from a macrophage-like mouse cell line, J774.1. Unlike the parental J774.1 cells, these mutants grew even in LPS added medium as well as in normal growth medium without any morphological changes. Assay of 125I-LPS binding to the cell monolayers revealed that one of these LPS-resistant mutants (LR-9) was strikingly defective in LPS-binding activity. Scatchard plot showed that LR-9 cells lacked the high affinity… CONTINUE READING

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