Isolation of a human kappa opioid receptor cDNA from placenta.

  title={Isolation of a human kappa opioid receptor cDNA from placenta.},
  author={Erik M{\aa}nsson and Lance Bare and Dongki Yang},
  journal={Biochemical and biophysical research communications},
  volume={202 3},
By using a mouse delta opioid receptor cDNA as a probe, a kappa opioid receptor gene was isolated from a human genomic library. Reverse transcriptase/PCR was subsequently used to isolate the corresponding cDNA from human placenta mRNA. Characterization of the cloned receptor with a kappa agonist in transfected COS-7 cells, revealed a Kd of 0.67 nM, which is similar to rodent kappa 1 receptors. In competition binding experiments, non-kappa agonists had kis > 1000nM. Furthermore, when expressed… CONTINUE READING
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