Isolation of a highly clonogenic and multipotential subfraction of adult stem cells from bone marrow stroma.

@article{Smith2004IsolationOA,
  title={Isolation of a highly clonogenic and multipotential subfraction of adult stem cells from bone marrow stroma.},
  author={Jason R. Smith and Radhika R. Pochampally and Anthony J Perry and S. C. Hsu and D. J. Prockop},
  journal={Stem cells},
  year={2004},
  volume={22 5},
  pages={823-31}
}
Attempts have been made to develop cell and gene therapies using the adult stem cells from bone marrow referred to as mesenchymal stem cells or marrow stromal cells (MSCs). However, the results have been variable in part because there are no standardized protocols for preparing and characterizing MSCs. In the experiments presented here, we developed a standardized assay by light scattering to measure the content of rapidly self-renewing cells (RS cells) in preparations of MSCs. The assay… CONTINUE READING