Isolation of a gramicidin S hyperproducing strain of Bacillus brevis by use of a flourescence activated cell sorting system

@article{Azuma1992IsolationOA,
  title={Isolation of a gramicidin S hyperproducing strain of Bacillus brevis by use of a flourescence activated cell sorting system},
  author={Takachika Azuma and Gail I. Harrison and Arnold L. Demain},
  journal={Applied Microbiology and Biotechnology},
  year={1992},
  volume={38},
  pages={173-178}
}
A gramicidin S (GS) hyperproducing mutant of Bacillus brevis was isolated by using a protein-staining flourescence dye (fluorescein isothiocyanate, FITC), and a fluorescence-activated cell sorting system (FACS). By flow cytometry (FCM) analysis after staining with FITC, higher producing cells of the wild-type had higher fluorescence signals than cells with low productivity or cells from a GS non-producing mutant. Staining with FITC did not affect the viability of cells under the conditions… CONTINUE READING

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