Isolation of a Lactobacillus salivarius Strain and Purification of Its Bacteriocin, Which Is Inhibitory to Campylobacter jejuni in the Chicken Gastrointestinal System

@article{Stern2006IsolationOA,
  title={Isolation of a Lactobacillus salivarius Strain and Purification of Its Bacteriocin, Which Is Inhibitory to Campylobacter jejuni in the Chicken Gastrointestinal System},
  author={Norman J. Stern and Edward A Svetoch and Boris V. Eruslanov and Vladimir V. Perelygin and E. V. Mitsevich and Irina P. Mitsevich and Victor D. Pokhilenko and Vladimir P. Levchuk and Olga E. Svetoch and Bruce S. Seal},
  journal={Antimicrobial Agents and Chemotherapy},
  year={2006},
  volume={50},
  pages={3111 - 3116}
}
ABSTRACT We evaluated anti-Campylobacter jejuni activity among >1,200 isolates of different lactic acid bacteria. Lactobacillus salivarius strain NRRL B-30514 was selected for further study. The cell-free, ammonium sulfate precipitate from the broth culture was termed the crude antimicrobial preparation. Ten microliters of the crude preparation created a zone of C. jejuni growth inhibition, and growth within the zone resumed when the crude preparation was preincubated with proteolytic enzymes… Expand
Isolation of Lactobacillus salivarius 1077 (NRRL B-50053) and Characterization of Its Bacteriocin, Including the Antimicrobial Activity Spectrum
TLDR
The therapeutic benefit of bacteriocin L-1077 was demonstrated in market-age broiler chickens colonized with both C. jejuni and Salmonella enterica serovar Enteritidis and appears to hold promise in controlling C.Jejuni/S.Enteritidis among commercial broiler birds. Expand
Purification and Characterization of a Novel Anti-Campylobacter Bacteriocin Produced by Lactobacillus curvatus DN317
TLDR
The antimicrobial activity of the bacteriocin, namely curvaticin DN317, was found to be bacteriostatic against Campylobacter jejuni ATCC 33560, which constitutes a reasonable advance in the antimicrobial field because of its potential applications in food technology. Expand
Purification of bacteriocin LS1 produced by human oral isolate Lactobacillus salivarius BGHO1.
TLDR
The purification of a bacteriocin, named LS1, produced by L. salivarius BGHO1 enabled the further characterization of LS1 on both the molecular and genetic levels, implying that LS1 is most probably a new bacteriOCin, different from previously described bacteriockins produced by lactobacillus strains. Expand
Selection and Characterization of the Bacteriocin-Producing Bacterium, Bacillus subtilis BP6 Isolated from Chicken Gut against Salmonella gallinarum Causing Fowl-typhus
TLDR
B. subtilis BP6 could be a non-antibiotic probiotic for prevention of chicken or duck typhus and antimicrobial activity of purified bacteriocin BP6 was lost, whereas that of n-butanol extract of non-peptide antibiotic-like substances was lost. Expand
A novel bacteriocin from Lactobacillus salivarius against Staphylococcus aureus: Isolation, purification, identification, antibacterial and antibiofilm activity
Abstract Bacteriocins are known to inhibit various foodborne bacteria and their biofilms; however, few bacteriocins with activity against food-derived Staphylococcus aureus have been reported. Here,Expand
Identification of lactic acid bacteria from poultry feces: evidence on anti-campylobacter and anti-listeria activities.
TLDR
The results of proteolytic, heat, and neutralizing treatments of supernatants from the aforementioned strains can conclude that antagonism observed is attributed to antimicrobial peptide or bacteriocin in the case of strain S37, whereas it is ascribable to a nonbacteriOCin, likely a reuterin, in the cases of strains S42 and S42. Expand
Bacteriocin-producing lactic acid bacteria as a probiotic potential from Thai indigenous chickens
TLDR
Bacteriocin-producing lactic acid bacteria (LAB) were isolated and screened from the gastrointesti- nal tract (GIT) of Thai indigenous chickens and the primary probiotic properties were determined. Expand
Purification and characterization of a new bacteriocin active against Campylobacter produced by Lactobacillus salivarius SMXD51.
TLDR
Strain SMXD51, isolated from chicken ceca and identified as Lactobacillus salivarius, produced a component that inhibits the growth of Gram-positive and Gram-negative bacteria and especially Campylobacter jejuni, which appears potentially very useful to reduce CampylOBacter in poultry prior to processing. Expand
Screening of adhesive lactobacilli with antagonistic activity against Campylobacter jejuni
TLDR
It is suggested that Lactobacillus strains N8, N9, ZL4 and ZL5 could be used as potential probiotics in food applications against C. jejuni infection. Expand
Bacteriocinogenic Lactobacillus plantarum ST16Pa isolated from papaya (Carica papaya) — From isolation to application: Characterization of a bacteriocin
TLDR
This is the first report on detection of L. plantarum in papaya through biochemical tests, PCR with species-specific primers and 16S rDNA sequencing and effect of MRS medium components, different initial pH and additions of glycerol or vitamins to the media on bacteriocin ST16Pa production was studied. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 46 REFERENCES
Isolation of Bacillus circulans and Paenibacillus polymyxa strains inhibitory to Campylobacter jejuni and characterization of associated bacteriocins.
TLDR
Anti-Campylobacter activity among 365 Bacillus and Paenibacillus isolates from poultry production environments was evaluated and antagonists and the corresponding bacteriocins may be useful in the control of Campylobacteria infection in poultry. Expand
Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus.
TLDR
Examination of strains of L. acidophilus within the six homology groupings of Johnson et al. demonstrated that production of the bacteriocin lactacin B could not be used in classification of neotype L. Acidophilus strains, however, the usefulness of employing sensitivity to lactac in B in classified of dairy lactobacilli is suggested. Expand
Isolation and characterization of acidocin A and cloning of the bacteriocin gene from Lactobacillus acidophilus
Acidocin A, a bacteriocin produced by Lactobacillus acidophilus TK9201, is active against closely related lactic acid bacteria and food-borne pathogens including Listeria monocytogenes. TheExpand
Properties of a Lactobacillus fermenti bacteriocin.
TLDR
The properties of a bacteriocin derived from Lactobacillus fermenti strain 466 were investigated and it was present in low titre in supernatant fluids from overnight broth cultures and was not inducible by ultraviolet radiation. Expand
Isolation, partial purification and characterization of a bacteriocin produced by a newly isolated Bacillus subtilis strain
TLDR
Its sensitivity to gastric peptidases and activity against the food‐borne pathogens make this bacteriocin potentially useful as an antimicrobial agent in foods. Expand
Comparative Studies of Class IIa Bacteriocins of Lactic Acid Bacteria
TLDR
The antagonistic activity of pediocin-PA1 and enterocin A was much more sensitive to reduction of disulfide bonds than the antagonisticactivity of sakacin P and curvacin A, suggesting that an extra disulfides bond that is present in the former two may contribute to their high levels of activity. Expand
Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481
TLDR
The data confirmed that antimicrobial activity of L. helveticus 481 was mediated by a bacteriocin, designated helveicin J, and provided evidence for a chromosomal location of helveticsin J and host immunity determinants. Expand
Bacteriocins of gram-positive bacteria.
In recent years, a group of antibacterial proteins produced by gram-positive bacteria have attracted great interest in their potential use as food preservatives and as antibacterial agents to combatExpand
Paenibacillus polymyxa purified bacteriocin to control Campylobacter jejuni in chickens.
TLDR
Feeding bacteriocins before poultry slaughter appears to provide control of C. jejuni to effectively reduce human exposure, and this advance is directed toward on-farm control of pathogens, as opposed to the currently used chemical disinfection of contaminated carcasses. Expand
Antagonistic Action of Lactobacillus acidophilus Toward Intestinal and Foodborne Pathogens in Associative Cultures 1.
Lactobacillus acidophilus exerted antagonistic actions on growth of Staphylococcus aureus , Salmonella typhimurium , enteropathogenic Escherichia coli , and Clostridium perfringens when grown withExpand
...
1
2
3
4
5
...