Isolation of a 250 million-year-old halotolerant bacterium from a primary salt crystal

@article{Vreeland2000IsolationOA,
  title={Isolation of a 250 million-year-old halotolerant bacterium from a primary salt crystal},
  author={Russell H. Vreeland and William D. Rosenzweig and Dennis W. Powers},
  journal={Nature},
  year={2000},
  volume={407},
  pages={897-900}
}
Bacteria have been found associated with a variety of ancient samples, however few studies are generally accepted due to questions about sample quality and contamination. When Cano and Borucki isolated a strain of Bacillus sphaericus from an extinct bee trapped in 25–30 million-year-old amber, careful sample selection and stringent sterilization techniques were the keys to acceptance. Here we report the isolation and growth of a previously unrecognized spore-forming bacterium (Bacillus species… Expand
New evidence for 250 Ma age of halotolerant bacterium from a Permian salt crystal
The purported oldest living organism, the spore-forming bacterium Virgibacillus sp. Perm- ian strain 2-9-3, was recently cultured from a brine inclusion in halite of the 250 Ma Permian SaladoExpand
The Permian bacterium that isn't.
TLDR
The proof-of- the-pudding-is-in-the-eating principle is applied to test whether the newly reported B. permians 16S ribosomal RNA gene sequence is ancient or not. Expand
The complete genome of a viable archaeum isolated from 123-million-year-old rock salt.
TLDR
This study is the first one to publish the complete genome of an organism originating from surface-sterilized ancient halite, and can become a key factor in understanding the mechanisms by which these organisms are able to survive in harsh conditions deep underground or possibly on other celestial bodies. Expand
Spore-forming halophilic bacteria isolated from Arctic terrains: Implications for long-range transportation of microorganisms
TLDR
This research implies the long-range transportation of halophilic bacteria from Arctic terrains to locations such as the Arctic by the wind. Expand
Isolation of Halobacterium salinarum retrieved directly from halite brine inclusions.
TLDR
The described method of retrieving particle-containing brine from fluid inclusions offers a robust approach for assessing the antiquity of microorganisms associated with evaporites. Expand
Halophilic Archaea Cultivated from Surface Sterilized Middle-Late Eocene Rock Salt Are Polyploid
TLDR
The polyploidy of halophilic archaea living in ancient rock salt might be a factor explaining how these organisms are able to overcome the challenge of prolonged survival during their entombment. Expand
Micrococcus luteus - Survival in Amber
TLDR
Evidence is demonstrated supporting the view that non-spore-forming cocci from a 120-million-year-old block of amber have numerous adaptations for survival in extreme, nutrient-poor environments, traits that will assist in this bacteria’s persistence and dispersal in the environment. Expand
Fatty acid and DNA analyses of Permian bacteria isolated from ancient salt crystals reveal differences with their modern relatives
TLDR
Comparisons between the Permian microbes and other isolates to which they appear related show that these bacteria are not identical and help to rule out questions of contamination by putatively modern strains. Expand
Halophilic Archaea cultured from ancient halite, Death
TLDR
Halophilic Archaea cultured from ancient fluid inclinations in a 90-m-long salt core from Death Valley, California, demonstrate survival of bacterial cells in subsurface halite for up to 34 000 years. Expand
Characterization of ancient DNA supports long-term survival of Haloarchaea.
TLDR
This work uses amplification, cloning, and sequencing of 16S ribosomal DNA to investigate the authenticity of halophilic archaea cultured from subsurface halite, Death Valley, California, 22,000 to 34,000 years old and provides the best independent support to date for the long-term survival of halophile archaea in ancient halite. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 49 REFERENCES
Development of a Protocol to Retrieve Microorganisms from Ancient Salt Crystals
Previous research has indicated that halophilic microorganisms are associated with salt crystals from ancient formations. However,this research has generally failed to convincingly demonstrate thatExpand
Diversity of Microorganisms Isolated from Amber
TLDR
The culture of bacteria from Dominican and previously untested 120 million year old Israeli (Lebanese lode) amber is reported and the internal topography of an amber slice is mapped. Expand
Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea.
TLDR
The isolates isolated from enrichments prepared from Dead Sea water samples collected 57 years ago should be regarded as members of a new species of the genus Bacillus, for which the name Bacillus marismortui sp. Expand
Halococcus salifodinae sp. nov., an Archaeal Isolate from an Austrian Salt Mine
TLDR
A novel extremely halophilic archaeon (archaebacterium) was isolated from rock salt obtained from an Austrian salt mine and it is proposed that this isolate should be recognized as a new species and should be named Halococcus salifodinae. Expand
Halobacteria: the evidence for longevity
TLDR
Results to date have yet to show any gene sequence differences between surface and evaporite-derived halobacteria that might arguably be an indication of long-term dormancy, which would predict that halob bacteria that have been insulated and isolated inside ancient evaporites would be different from comparable bacteria from surface environments. Expand
Distribution and diversity of halophilic bacteria in a subsurface salt formation
TLDR
A biochemical comparison between ten known species of halophilic bacteria, and strains isolated from both the mine and nearby surface hypersaline lakes, showed the presence of extreme halophiles with wide biochemical diversity, some of which could prove to represent previously undescribed groups. Expand
Revival and identification of bacterial spores in 25- to 40-million-year-old Dominican amber.
TLDR
A bacterial spore was revived, cultured, and identified from the abdominal contents of extinct bees preserved for 25 to 40 million years in buried Dominican amber that indicated that the ancient bacterium is most closely related to extant Bacillus sphaericus. Expand
Bacillus salexigens sp. nov., a new moderately halophilic Bacillus species.
TLDR
Six moderately halophilic, grampositive, rod-shaped strains isolated from salterns and hypersaline soils located in different geographical areas of Spain constitute a homology group with levels of DNA-DNA homology ranging from 73 to 100%. Expand
Staphylococcus succinus sp. nov., isolated from Dominican amber.
TLDR
The results indicate that AMg-D1T and AMG-D2 represent a novel species, which was named Staphylococcus succinus sp. Expand
Virgibacillus : a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus
TLDR
The species Virgibacillus pantothenticus and related organisms comprising this new genus can be distinguished fromMembers of Bacillus rRNA group 1 (Bacillus sensu stricto), and from members of Paenibacillin and other aerobic endospore-forming bacteria by routine phenotypic tests. Expand
...
1
2
3
4
5
...