Isolation of a 250 million-year-old halotolerant bacterium from a primary salt crystal

@article{Vreeland2000IsolationOA,
  title={Isolation of a 250 million-year-old halotolerant bacterium from a primary salt crystal},
  author={Russell H. Vreeland and William D. Rosenzweig and Dennis W. Powers},
  journal={Nature},
  year={2000},
  volume={407},
  pages={897-900}
}
Bacteria have been found associated with a variety of ancient samples, however few studies are generally accepted due to questions about sample quality and contamination. When Cano and Borucki isolated a strain of Bacillus sphaericus from an extinct bee trapped in 25–30 million-year-old amber, careful sample selection and stringent sterilization techniques were the keys to acceptance. Here we report the isolation and growth of a previously unrecognized spore-forming bacterium (Bacillus species… 
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