Isolation of Theileria sergenti piroplasms from infected erythrocytes and development of an enzyme-linked immunosorbent assay for serodiagnosis of T sergenti infections.

Abstract

A method of obtaining a pure suspension of Theileria sergenti piroplasms from infected bovine erythrocytes was developed and the resulting parasites used as antigen in an enzyme-linked immunosorbent assay (ELISA). Piroplasms were freed from infected erythrocytes using the nitrogen cavitation technique and then separated from unbroken erythrocytes by… (More)

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