Isolation of Sperm Cells from the Pollen of Plumbago zeylanica.

@article{Russell1986IsolationOS,
  title={Isolation of Sperm Cells from the Pollen of Plumbago zeylanica.},
  author={Scott D Russell},
  journal={Plant physiology},
  year={1986},
  volume={81 1},
  pages={317-9}
}
Intact sperm cells of Plumbago zeylanica were released from mature pollen grains near anthesis using osmotic shock with 20% sucrose. Sperm cell yields of up to 75% can be attained by differential centrifugation using a clinical centrifuge with concentrations of up to 8.8 x 10(6) cells/milliliter. Such ;gametoplasts' remain intact for up to 24 hours according to Evan's blue exclusion and can be used for characterization or physiological manipulation.