Isolation of Rickettsia rhipicephali and Rickettsia bellii from Haemaphysalis juxtakochi Ticks in the State of São Paulo, Brazil

@article{Labruna2006IsolationOR,
  title={Isolation of Rickettsia rhipicephali and Rickettsia bellii from Haemaphysalis juxtakochi Ticks in the State of S{\~a}o Paulo, Brazil},
  author={Marcelo Bahia Labruna and Richard de Campos Pacheco and Leonardo Jos{\'e} Richtzenhain and Mat{\'i}as Pablo Juan Szab{\'o}},
  journal={Applied and Environmental Microbiology},
  year={2006},
  volume={73},
  pages={869 - 873}
}
ABSTRACT In the present study, attempts to isolate Rickettsia in cell culture were performed individually in seven specimens of Haemaphysalis juxtakochi ticks collected in the state of São Paulo (southeastern Brazil). Rickettsia was successfully isolated by the shell vial technique and established in Vero cell culture from six ticks (six isolates). DNA extracted from infected cells of these isolates was tested by PCR and DNA sequencing, using genus-specific Rickettsia primers targeting the… Expand
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