Isolation of RNA of high quality and yield from Ginkgo biloba leaves

@article{Wang2005IsolationOR,
  title={Isolation of RNA of high quality and yield from Ginkgo biloba leaves},
  author={T. W. Wang and Nianhui Zhang and Lingfang Du},
  journal={Biotechnology Letters},
  year={2005},
  volume={27},
  pages={629-633}
}
An improved protocol was developed to isolate total RNA in good yield and integrity from Ginkgo biloba leaves containing high levels of flavonoid glycosides, terpene lactones, carbohydrates and polyphenolic secondary metabolites. Polyvinylpolypyrrolidone at 2% and β-mercaptoethanol at 4% were added to the standard CTAB extraction buffer and, after chloroform and phenol extraction, the pellet obtained by ethanol/acetate precipitation was washed and a second phenol/chloroform extraction was… CONTINUE READING