Isolation of RNA aptamers specific to the NS3 protein of hepatitis C virus from a pool of completely random RNA.

@article{Kumar1997IsolationOR,
  title={Isolation of RNA aptamers specific to the NS3 protein of hepatitis C virus from a pool of completely random RNA.},
  author={Penmetcha K R Kumar and Koudai Machida and Petri T Urvil and Nobuko Kakiuchi and D Vishnuvardhan and Kunitada Shimotohno and Kazunari Taira and Satoshi Nishikawa},
  journal={Virology},
  year={1997},
  volume={237 2},
  pages={
          270-82
        }
}
Hepatitis C virus (HCV) is a single-stranded RNA virus and its genome is translated into a single large polyprotein. The viral-encoded NS3 protein possesses protease, nucleoside triphosphatase, and helicase activities. Since these activities appear to be important for viral replication, efforts are being made to identify compounds that might inhibit the enzymatic activities of NS3 and serve as potential anti-HCV agents. We used a genetic selection strategy in vitro to isolate, from a pool of… CONTINUE READING

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