Isolation of Nucleoli of the Walker Carcinosarcoma and Liver of the Rat following Nuclear Disruption in a French Pressure Cell.

Abstract

A method is presented for the isolation of nucleoli of the rat liver and the Walker tumor in which the tissues were initially homogenized in a solution containing 0.25 M sucrose and 0.005 M CaCl2 and then were compressed and rapidly decompressed in a French pressure cell. Approximately 60 minutes after homogenization the sample was subjected to 5000-7000 p… (More)

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